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Subtyping of group specific component (GC) in microbloodstains and semen stains by isoelectric focusing in ultrathin immobilized pH gradient gels followed by enzyme immunodetection
Author(s) -
Pflug Werner
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090818
Subject(s) - subtyping , isoelectric focusing , chromatography , chemistry , stain , isoelectric point , enzyme , biochemistry , staining , biology , computer science , genetics , programming language
A powerful method for group specific component (GC) subtyping with good resolution of GC bands by isoelectric focusing on ultrathin immobilized pH gradients is described. After separation, GC detection is achieved using a highly sensitive alkaline phosphatase conjugated enzyme‐immuno system. The efficiency of the method in forensic case work for subtyping GC from diluted bloodstain extracts, blood microstains and semen stains is demonstrated. Furthermore, GC subtyping and selective detection of human GC provides the evidence for human origin of the stain. This is important when analyzing microstains with limited stain consumption.