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Subtyping of phosphoglucomutase 1 (PGM1) in microstains of different biological materials with subsequent species differentiation without further stain material consumption
Author(s) -
Pflug Werner
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090817
Subject(s) - phosphoglucomutase , stain , isoelectric focusing , subtyping , biology , silver stain , staining , chromatography , biochemistry , chemistry , microbiology and biotechnology , genetics , computer science , programming language , enzyme
Micromethods for subtyping of phosphoglucomutase 1 (PGM 1) in small amounts of biological stain material are described, using an applicator for highly diluted stain extracts. With the aid of this applicator strip blood and semen micro‐stains as well as single hair‐roots could be extracted by electroelution directly on the PGM 1 isoelectric focusing gel. Species differentiation was also possible either by radial immunodiffusion using the extract remaining in the applicator strip after isoelectric focusing or by interpretation of the PGM pattern itself.