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Subtyping of orosomucoid 1 (ORM1) by isoelectric focusing in agarose and polyacrylamide gels
Author(s) -
Thymann Mariann,
Weidinger Sebastian
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090806
Subject(s) - orosomucoid , isoelectric focusing , subtyping , immunofixation , agarose , locus (genetics) , population , chemistry , polyacrylamide gel electrophoresis , allele , genetics , biology , chromatography , gene , biochemistry , glycoprotein , enzyme , medicine , computer science , antibody , monoclonal antibody , monoclonal , programming language , environmental health
Genetic polymorphism of orosomucoid (ORM) has been demonstrated in several populations and comprises two structural gene loci, ORM 1 and ORM2. In Caucasians three common ORM 1 alleles have been shown, while the ORM 2 locus is almost monomorphic. ORM 1 phenotyping by isoelectric focusing in agarose or polyacrylamide gel combined with either print immunofixation or enzyme‐linked immunoblotting is described, and population and family data from Denmark and Southern Germany are given. It is proposed to use a different alpha‐numerical nomenclature for the phenotypes of the ORM 1 and ORM 2 systems.