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Optimized oligonucleotide probes for DNA fingerprinting
Author(s) -
Schäfer Renate,
Zischler Hans,
Birsner Uli,
Becker Andrea,
Epplen Jörg T.
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090804
Subject(s) - oligonucleotide , dna profiling , biology , microbiology and biotechnology , dna , restriction enzyme , hybridization probe , genetics , molecular probe , oligomer restriction
The three different simple repetitive oligonucleotide probes (CT) 8 , (CAC) 5 and (TCC) 5 were hybridized to a panel of human DNAs which had been digested with the restriction endonucleases Alu I, Hinf I and Mbo I. The resulting DNA fingerprints were analyzed and different parameters calculated, such as the maximal mean allele frequency and the average number of polymorphic bands per individual. The highest number of bands was obtained after hybridization of Hinf I digested DNA with (CAC) 5 . The probability of finding the same band pattern as in individual A in individual B is 2 × 10 −8 . The DNAs of monozygous twins show indistinguishable banding patterns and the bands are inherited according to the Mendelian laws. Thus this procedure reveals informative fingerprints that can be used for individual identification, e. g. in paternity testing and in forensic applications. In most of these experiments 32 P‐labelled probes were employed, yet the biotinylated oligonucleotide (GACA) 4 produced results which were equivalent to those obtained by hybridization with the 32 P‐labelled probe (GACA) 4 .