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Specific antigen detection by immunoelectroendosmosis
Author(s) -
Lewis W. H. P.,
Sun K. K. Y.
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090713
Subject(s) - antigen , antibody , peroxidase , specific antibody , stage (stratigraphy) , biology , immunology , chemistry , microbiology and biotechnology , biochemistry , enzyme , paleontology
A novel approach to the immunological stage of immunoblotting is described. The method employs the phenomenon of electroendosmosis to bring the antibodies sequentially into contact with the antigen. Using a three‐stage peroxidase antiperoxidase system for human group specific protein (Gc) phenotyping, it has been shown that the method has comparable sensitivity, is more rapid and is more economical of antibodies than a similar multi‐stage immerse‐and‐wash system.