A simple method for preparation of molecular weight marker proteins for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis by photopolymerizations of hemoglobin subunits | Zendy

Sato Hiroshi | Zendy; Aono Sachiko | Zendy; Semba Reiji | Zendy; Kashiwamata Shigeo | Zendy

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A simple method for preparation of molecular weight marker proteins for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis by photopolymerizations of hemoglobin subunits

Author(s) -

Sato Hiroshi,

Aono Sachiko,

Semba Reiji,

Kashiwamata Shigeo

Publication year - 1988

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150090712

Subject(s) - sodium dodecyl sulfate , hemoglobin , molecular mass , chemistry , polyacrylamide gel electrophoresis , globin , gel electrophoresis , polyacrylamide , dimer , polymer , chromatography , biochemistry , polymer chemistry , organic chemistry , enzyme

Photoirradiation of globin, obtained from human hemoglobin, in the presence of oxygen and protoporphyrin produced a series of polymers of globin subunits ranging from dimer to dodecamer. These polymers are useful as molecular weight markers because they cover a relatively wide range of molecular weights ( M r 15 500–186 000) with a constant and narrow interval of M r 15 500.

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