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A simple method for preparation of molecular weight marker proteins for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis by photopolymerizations of hemoglobin subunits
Author(s) -
Sato Hiroshi,
Aono Sachiko,
Semba Reiji,
Kashiwamata Shigeo
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090712
Subject(s) - sodium dodecyl sulfate , hemoglobin , molecular mass , chemistry , polyacrylamide gel electrophoresis , globin , gel electrophoresis , polyacrylamide , dimer , polymer , chromatography , biochemistry , polymer chemistry , organic chemistry , enzyme
Photoirradiation of globin, obtained from human hemoglobin, in the presence of oxygen and protoporphyrin produced a series of polymers of globin subunits ranging from dimer to dodecamer. These polymers are useful as molecular weight markers because they cover a relatively wide range of molecular weights ( M r 15 500–186 000) with a constant and narrow interval of M r 15 500.