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Rapid imaging, using a cooled charge‐coupled‐device, of fluorescent two‐dimensional polyacrylamide gels produced by labelling proteins in the first‐dimensional isoelectric focusing gel with the fluorophore 2‐methoxy‐2,4‐diphenyl‐3(2H)furanone
Author(s) -
Jackson Peter,
Urwin Valerie E.,
MacKay Craig D.
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090709
Subject(s) - fluorophore , isoelectric focusing , labelling , fluorescence , polyacrylamide , polyacrylamide gel electrophoresis , charge coupled device , chemistry , chromatography , materials science , analytical chemistry (journal) , optics , physics , biochemistry , polymer chemistry , enzyme
A new method for visualising proteins in two‐dimensional polyacrylamide gels was developed. Proteins were labelled with the fluorophore 2‐methoxy‐2,4‐diphenyl‐3(2H)furanone (MDPF) while present in the first‐dimensional gel after isoelectric focusing and subsequently electrophoresed into the second‐dimensional gel. High resolution spot patterns were produced and compared with other methods of visualisation. A new rapid imaging system based on a cooled charge‐coupled‐device was used to view the two‐dimensional fluorescent protein spot patterns. The method allows the immediate and rapid imaging of two‐dimensional gels at the end of electrophoresis with no further processing.