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Comparative study of electrophoretic patterns of latex proteins from clones of Hevea brasiliensis
Author(s) -
Arreguín Barbarín,
Lara Paulina,
Rodríguez Roberto
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090707
Subject(s) - hevea brasiliensis , sodium dodecyl sulfate , hevea , chromatography , densitometry , electrophoresis , gel electrophoresis , chemistry , natural rubber , polyacrylamide gel electrophoresis , microbiology and biotechnology , biology , biochemistry , medicine , organic chemistry , enzyme
Latex serum proteins from Hevea brasiliensis were studied by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Proteins from whole serum and fractions isolated by gel chromatography on Ultrogel AcA 44 were analyzed. No qualitative clonal differences were found in the protein patterns of whole latex or in the fractions but laser densitometry revealed reliable quantitative differences in protein composition. Reproducible mobilities and molecular weights of selected bands were obtained both within single gels as well as in different gels, analyzing severallots of latex received at various times from a Hevea experimental field station. The clones compared were IAN 710, GV 31, GV 42; the first one had the highest rubber yields.