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Comparison between isoelectric focusing methods for the detection of orosomucoid phenotypes
Author(s) -
Montiel Maria Dolores,
Carracedo Angel,
LopezRodriguez Isidro,
RodriguezCalvo Maria Sol,
Concheiro Luis,
Huguet Emilio,
Gené Manuel
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090605
Subject(s) - isoelectric focusing , immunofixation , orosomucoid , population , lectin , isoelectric point , chromatography , chemistry , biology , biochemistry , microbiology and biotechnology , genetics , glycoprotein , medicine , monoclonal , environmental health , monoclonal antibody , enzyme , antibody
Orosomucoid (ORM) polymorphism was investigated by different methods including isoelectric focusing in acid pH ranges followed by silver staining, print immunofixation of desialyzed ORM, fixation using a lectin from the sea‐weed Codium tomentosum , isoelectric focusing followed by immunofixation in miniaturized gels and isoelectric focusing in immobilized pH gradients. Population genetics studies were carried out in Galicia (NW Spain) and two new ORM variants were found.