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Linear Ferguson plots of polystyrene sulfate size standards for the quantitative agarose gel electrophoresis of subcellular particles
Author(s) -
Hahn Elisabeth,
Wurts Linda,
Tietz Dietmar,
Chrambach Andreas
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090602
Subject(s) - agarose , polystyrene , chromatography , chemistry , agarose gel electrophoresis , electrophoresis , color marker , sulfate , analytical chemistry (journal) , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , polymer , dna , enzyme , organic chemistry
Accurately standardized commercial polystyrene sulfate particles in agarose gel electrophoresis yield linear Ferguson plots at pH 7.4 over a gel concentration range up to 0.9% agarose which do not exhibit any significant sigmoidal curve elements, using either a discontinuous buffer system or a continuous buffer. Ferguson plots of these standard‐sized particles were evaluated using alternatively a linear or convex model, by means of a newly developed set of programs (to be used in conjunction with program M‐LAB) which (i) is sufficiently user‐friendly to allow for quantitative agarose gel electrophoresis of subcellular‐sized spherical particles based on their convex Ferguson plots with the same operational simplicity previously available for linear Ferguson plots only; (ii) simultaneously and interactively analyzes the Ferguson plots of all particles under consideration on the basis of an extended Ogston model.