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A comparative study of protein synthesis by keratinocytes and fibroblasts in vitro using two‐dimensional gel electrophoresis and dual isotope autoradiography
Author(s) -
Easty David J.,
Patel Ketan,
Dover Robin,
Evans David J.,
Dunn Michael J.
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090507
Subject(s) - keratinocyte , in vitro , two dimensional gel electrophoresis , gel electrophoresis , microbiology and biotechnology , polyacrylamide gel electrophoresis , methionine , keratin , biology , biochemistry , chemistry , proteomics , amino acid , gene , enzyme , genetics
A procedure is described for the detection of keratinocyte specific proteins. Fibroblasts and keratinocytes were isolated from human skin and radiolabelled in vitro. Samples were separated by two‐dimensional polyacrylamide gel electrophoresis to compare the proteins synthesised by the different types of cultured skin cells. Dual label autoradiography of samples radiolabelled with [ 35 S]methionine and [ 75 Se]selenomethionine was used to identify keratinocyte specific proteins. We report 45 keratinocyte‐specific components and identify some of these proteins. The differential expression of these proteins and their relevance to epidermal differentiation are discussed.