Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles | Zendy

Orbán László | Zendy; Hahn Elisabeth | Zendy; Chrambach Andreas | Zendy

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Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles

Author(s) -

Orbán László,

Hahn Elisabeth,

Chrambach Andreas

Publication year - 1988

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150090404

Subject(s) - agarose , ethidium bromide , gel electrophoresis of nucleic acids , buffer (optical fiber) , color marker , agarose gel electrophoresis , electrophoresis , chromatography , chemistry , dna , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , computer science , gel electrophoresis of proteins , enzyme , telecommunications

Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 °C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre‐stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.

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