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PI Mtoyoura: A new PI M subtype found by separator and hybrid isoelectric focusing
Author(s) -
Yuasa Isao,
Suenaga Kazuyuki,
Umetsu Kazuo,
Inagaki Osamu,
Ikebuchi Jun,
Ito Keiichi,
Okada Kichiro
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090311
Subject(s) - isoelectric focusing , pi , isoelectric point , separator (oil production) , chemistry , population , chromatography , genotype , gene , analytical chemistry (journal) , microbiology and biotechnology , biology , biochemistry , physics , enzyme , medicine , thermodynamics , environmental health
In the course of a population study of α 1 ‐antitrypsin polymorphism by separator isoelectric focusing, a variant phenotype having a somewhat narrower spacing than PI M1M3 was observed in a Japanese blood donor. Family studies by hybrid isoelectric focusing in a carrier ampholyte‐supplemented immobilized pH gradient from 4.35–4.65 revealed that the products of the responsible gene, PI * Mtoyoura , were extremely close but slightly cathodal to those of PI * M1. The difference in isoelectric point between them corresponded to the resolving limit of isoelectric focusing. For this reason, although the propositus' father was deduced to have the genotype PI * M1 / PI * Mtoyoura , the products of these two genes failed to form a double band pattern. Thus, the gene frequency for PI * Mtoyoura was unknown. These findings, however, indicate that a further microheterogeneity in the PI M subtype exists at least in the Japanese.