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Demonstration and elimination of artifacts associated with gold staining of proteins on nitrocellulose membranes after separation by sodium dodecyl sulphatepolyacrylamide gel electrophoresis
Author(s) -
Bath M. L.
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090309
Subject(s) - nitrocellulose , sodium dodecyl sulfate , chromatography , chemistry , gel electrophoresis , sodium , staining , membrane , electrophoresis , polyacrylamide gel electrophoresis , biochemistry , biology , enzyme , organic chemistry , genetics
Artifacts associated with gold staining of polypeptides separated by sodium dodecyl sulphate – polyacrylamide gel electrophoresis and transferred to nitrocellulose are eliminated by alkylation of reduced samples or by deletion of the reducing agent.