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Isoelectric focusing of catalase from acatalasemic mouse and human blood, and cultured human skin fibroblasts
Author(s) -
Ogata Masana,
Satoh Yukinori
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090305
Subject(s) - catalase , isoelectric point , microbiology and biotechnology , isoelectric focusing , chemistry , fibroblast , chromatography , biochemistry , enzyme , biology , in vitro
Hemolysates of normal, heterozygous hypocatalasemic and acatalasemic mice and of Japanese acatalasemic subjects were separated into three fractions, A, B and C, by DEAE‐cellulose column chromatography, and p I values of A, B and C fractions were determined by isoelectric focusing. The p I value of catalase in the A, B and C fractions increased in the order of normal, hypocatalasemic and acatalasemic mouse blood. The results obtained from Japanese acatalasemic blood samples showed that the p I values of catalase in the A, B and C fractions were similar to those in normal blood. Catalase in Japanese acatalasemic cultured skin fibroblasts was also analyzed by isoelectric focusing. The p I values of catalase in the extract from the cultured skin acatalasemic fibroblasts was similar to that in normal fibroblasts.