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Low voltage isoelectric focusing for the study of red cell acid phosphatase polymorphism: Improved phenotyping and better agreement with the Hardy Weinberg distribution
Author(s) -
DestroBisol Giovanni,
Ranalletta Dalila
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090211
Subject(s) - isoelectric focusing , cellophane , microbiology and biotechnology , isoelectric point , polymorphism (computer science) , typing , population , incubation , acid phosphatase , chemistry , chromatography , biology , analytical chemistry (journal) , biochemistry , genetics , allele , enzyme , gene , medicine , environmental health , organic chemistry
A low voltage isoelectric focusing (IEF) method for typing human red cell acid phosphatase (ACPl) polymorphism is reported. A clear differentiation of the ACPl*A, ACPl*B and ACPl*C allelic products is obtained by minimizing diffusion of the bands during electrophoresis and during enzyme visualization by using a hydrophilic cellophane film soaked with the substrate solution, heated to 50 °C, and by reading without incubation in a moist chamber. A population study of ACPl, carried out with this method, on a sample of 624 unrelated individuals of the population of Rome (gene frequencies: ACPl*A = 0.267; ACPl*B = 0.680; ACPl*C = 0.053), showed a good agreement between the observed and expected phenotype distribution (χ 2 2= 1.35; 0.70 > p > 0.50).