A continuous acetie acid system for polyacrylamide gel electrophoresis of gliadins and other prolamines | Zendy

Clements Robert L. | Zendy

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A continuous acetie acid system for polyacrylamide gel electrophoresis of gliadins and other prolamines

Author(s) -

Clements Robert L.

Publication year - 1988

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150090206

Subject(s) - acetic acid , electrophoresis , lactic acid , polyacrylamide gel electrophoresis , chromatography , chemistry , polyacrylamide , gel electrophoresis of proteins , buffer (optical fiber) , gel electrophoresis , acrylamide , biochemistry , biology , bacteria , enzyme , organic chemistry , polymer , computer science , polymer chemistry , monomer , telecommunications , genetics

A polyacrylamide gel electrophoresis system buffered by acetic acid alone was developed for electrophoresis of prolamines. When applied to gliadin electrophoresis, the acetic acid system produces more bands than does a conventional aluminum lactate‐lactic acid system (using 12 % acrylamide gels). The acetic acid system is relatively simple, requiring a single buffer component that is universally available in high purity.

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