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Separation accuracy of free flow electrophoresis as proved by flow cytometry
Author(s) -
Bauer Johann,
Kachel Volker
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090113
Subject(s) - free flow electrophoresis , flow cytometry , electrophoresis , chromatography , cell , population , antibody , elutriation , biology , microbiology and biotechnology , chemistry , polyacrylamide gel electrophoresis , immunology , biochemistry , gel electrophoresis of proteins , enzyme , medicine , environmental health , organic chemistry
The separation accuracy of the free flow electrophoresis ACE 710 device was proved by immunological methods. Several cell populations of human peripheral blood were purified using physical cell isolation methods such as countercurrent centrifugal elutriation and cell electrophoresis. The purified cell fractions were treated with fluoresceinisothiocyanate‐labelled antibodies. The percentages of cells in each fraction binding the antibodies of interest were determined by flow cytometry. The analyses revealed that with the help of free flow electrophoresis, given cell population of human peripheral blood can be highly enriched and that preenrichment of minor cell populations enhances the efficacy of a flow cytometer.