Premium
Increased sensitivity for Coomassie staining of sodium dodecyl sulfate‐polyacrylamide gels using PhastSystem Development Unit
Author(s) -
Heukeshoven Jochen,
Dernick Rudolf
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090112
Subject(s) - staining , chemistry , sodium dodecyl sulfate , coomassie brilliant blue , chromatography , polyacrylamide , sodium , silver stain , detection limit , ammonium sulfate , acetic acid , biochemistry , microbiology and biotechnology , biology , organic chemistry , polymer chemistry , genetics
Staining of proteins in PhastGel gradient media with Coomassie Blue R 350 was considerably improved using a lower concentration of methanol (10% v/v) and 2% ammonium sulfate in the staining solution and 10% acetic acid for destaining. The detection limit in sodium dodecyl sulfate‐polyacrylamide gels was lowered by a factor of 10 to about 2 ng per protein band. The Coomassie staining method was adapted to the newly developed silver staining procedure so that both can be used in parallel in PhastSystem.