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Horizontal two‐dimensional electrophoresis with immobilized pH gradients using PhastSystem
Author(s) -
Görg Angelika,
Postel Wilhelm,
Günther Siegfried,
Friedrich Claudia
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090111
Subject(s) - electrophoresis , chromatography , chemistry , gel electrophoresis , sodium dodecyl sulfate , two dimensional gel electrophoresis , gel electrophoresis of proteins , immobilized ph gradient , polyacrylamide gel electrophoresis , analytical chemistry (journal) , biochemistry , enzyme , isoelectric focusing , proteomics , gene
Protocols for horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two‐dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first‐dimensional immobilized pH gradient gel strip prior to second‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis were evaluated. Silver stained two‐dimensional patterns were obtained within 3.5 h.