Premium
A simple, inexpensive method for the salt‐free concentration of proteins for analysis by two‐dimensional gel electrophoresis
Author(s) -
Maresh Grace A.,
Dunbar Bonnie S.
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090110
Subject(s) - simple (philosophy) , electrophoresis , chromatography , chemistry , gel electrophoresis , gel electrophoresis of proteins , salt (chemistry) , two dimensional gel electrophoresis , polyacrylamide gel electrophoresis , biological system , biology , biochemistry , proteomics , gene , enzyme , philosophy , epistemology
A rapid, inexpensive method for the salt‐free concentration of small quantities of proteins for analysis by two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) has been developed. Proteins adsorbed to diatomaceous earth are subsequently removed using either sodium dodecyl sulfate or urea solubilization reagents for 2D‐PAGE analysis. This procedure has been found to concentrate proteins having wide ranges of molecular weight and charge. It is also valuable for the concentration of large numbers of small samples from cells cultured in vitro .