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Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels
Author(s) -
Heukeshoven Jochen,
Dernick Rudolf
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090106
Subject(s) - staining , sodium thiosulfate , sodium dodecyl sulfate , silver stain , chemistry , chromatography , sodium , thiosulfate , detection limit , polyacrylamide , microbiology and biotechnology , biology , inorganic chemistry , sulfur , organic chemistry , polymer chemistry , genetics
A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05–0.1 ng protein per band. Total staining time with the new procedure is 75 min.