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Fast horizontal electrophoresis. I. Isoelectric focusing and polyacrylamide gel electrophoresis using PhastSystem™
Author(s) -
Olsson Ingmar,
AxiöFredriksson UllaBritt,
Degerman Margareta,
Olsson Birgitta
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090104
Subject(s) - isoelectric focusing , chromatography , electrophoresis , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , two dimensional gel electrophoresis , pulsed field gel electrophoresis , color marker , gel electrophoresis , chemistry , biochemistry , proteomics , enzyme , genotype , gene
PhastSystem, an integrated system for horizontal electrophoresis and isoelectric focusing in small gels, including automated staining and destaining, is described. Buffers for electrophoresis are supplied to the gel from buffer strips made of agarose. The separation bed is cooled by Peltier elements. All conditions of significance to the results, both during separation and development, are controlled by a microprocessor. For separation, nine programs are available, each with 9 steps; for developement, there are nine programs with 20 steps each. In this paper, we also report results using PhastSystem for purity checking and characterization of monoclonal antibodies after affinity chromatography and also for determining their digestion rate with papain.