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Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of human salivary proteins including pink‐violet components: Range, distribution and response to 2‐mercaptoethanol
Author(s) -
Marshall Thomas,
Williams Katherine M.
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150081208
Subject(s) - coomassie brilliant blue , staining , saliva , chromatography , sodium dodecyl sulfate , centrifugation , gel electrophoresis , polyacrylamide gel electrophoresis , urea , silver stain , chemistry , electrophoresis , sodium , microbiology and biotechnology , biochemistry , biology , enzyme , genetics , organic chemistry
Mid‐morning specimens of unstimulated whole saliva were collected from 160 apparently healthy individuals and analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis following sample preparation with or without 2‐mercaptoethanol. Coomassie Brilliant Blue R‐250 staining revealed blue and pink‐violet salivary protein components. The latter, detected in approximately 60 % of the individuals, formed a limited range of patterns combining 2–8 of 11 diffuse zones of M r 10 000–100 000. The pink‐violet components were unaffected by 2‐mercaptoethanol, urea or prior centrifugation of the sample and were not invariably present or absent in repeat samples from the same individual. The patterns of the blue staining components were more reproducible for the purpose of human individualisation but were affected by 2‐mercaptoethanol or prior centrifugation of the sample. The patterns of both the blue and pink‐violet components were not affected by sample storage at −70 °C. Only 5 μL of saliva were required for analysis giving excellent resolution over the M r range 10 000–500 000.