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A comparison of rapid staining techniques for one‐ and two‐dimensional polyacrylamide gels
Author(s) -
Patel Ketan,
Easty David,
Bailey Richard,
Dunn Michael J.,
Evans David
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150081009
Subject(s) - staining , coomassie brilliant blue , nitrocellulose , chromatography , polyacrylamide , polyacrylamide gel electrophoresis , chemistry , blot , microbiology and biotechnology , biochemistry , biology , membrane , enzyme , genetics , gene
Two rapid staining procedures using 8‐anilino‐1‐naphthalene sulphonate (ANS) and Nitro Blue Tetrazolium (NBT) were compared with a Coomassie Brilliant Blue R‐250 staining technique. The methods were applied to protein separations using both one‐ (1‐D) and two‐dimensional (2‐D) polyacrylamide gel electrophoresis. The ANS procedure worked well for both 1‐D and 2‐D gels. The NBT technique, although more rapid, gave satisfactory results only for 2‐D gels. The ANS and NBT staining methods were both compatible with Western blotting so that they could be used for visualisation of the separation profile prior to blot transfer to nitrocellulose.