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The application of ligand overlay of protein blots to the study of the nicotinic acetylcholine receptor
Author(s) -
Gershoni Jonathan M.
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080911
Subject(s) - acetylcholine receptor , blot , nicotinic acetylcholine receptor , nicotinic agonist , protein subunit , receptor , chemistry , ligand (biochemistry) , western blot , biochemistry , biology , microbiology and biotechnology , gene
Protein blotting is used to identify and characterize the ligand binding site of the nicotinic acetylcholine receptor. The affinity of the complex formed on the filter can be determined by quantitative blot analyses. The precise binding‐sequence can be pinpointed by toxin overlay of proteolysed receptor α‐subunit. These techniques are now being employed in the analysis of bacterially expressed toxin binding sites.