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Electrophoresis of ganglioside micelles
Author(s) -
Catsimpoolas Nicholas,
Griffith Ann L.
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080803
Subject(s) - micelle , ganglioside , chemistry , polyacrylamide gel electrophoresis , chromatography , monomer , gel electrophoresis , electrophoresis , aqueous solution , polyacrylamide , gel electrophoresis of proteins , organic chemistry , biochemistry , polymer chemistry , polymer , enzyme
Gangliosides are known to exist as monomers in organic solvents and to aggregate in aqueous systems with the formation of high molecular weight micelles. It is shown here that the ganglioside micelles can be rendered visible by incorporation of a lipophilic dye and analyzed by conventional polyacrylamide gel electrophoresis. Mixed micelles of gangliosides and commonly used detergents as well as interaction products of ganglioside micelles with albumin were thus visualized by polyacrylamide gel electrophoresis for the first time.