Alpha 1‐antitrypsin and orosomucoid phenotyping using allo A lectin‐peroxidase for detection | Zendy

Umetsu Kazuo | Zendy; Yuasa Isao | Zendy; Taketa Kazuhisa | Zendy; Suzuki Tsuneo | Zendy

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Alpha 1‐antitrypsin and orosomucoid phenotyping using allo A lectin‐peroxidase for detection

Author(s) -

Umetsu Kazuo,

Yuasa Isao,

Taketa Kazuhisa,

Suzuki Tsuneo

Publication year - 1987

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150080708

Subject(s) - orosomucoid , isoelectric focusing , lectin , neuraminidase , glycoprotein , microbiology and biotechnology , chemistry , isoelectric point , biology , biochemistry , enzyme

A new method of identifying allo A lectin‐binding glycoproteins separated by isoelectric focusing is described. The method is based on the combination of neuraminidase treatment, isoelectric focusing and Durapore filter blotting followed by detection with allo A lectin. The technique is simple and reproducible for the determination of both the alpha 1‐antitrypsin and orosomucoid phenotypes in genetic and forensic investigations.

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