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Alpha 1‐antitrypsin and orosomucoid phenotyping using allo A lectin‐peroxidase for detection
Author(s) -
Umetsu Kazuo,
Yuasa Isao,
Taketa Kazuhisa,
Suzuki Tsuneo
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080708
Subject(s) - orosomucoid , isoelectric focusing , lectin , neuraminidase , glycoprotein , microbiology and biotechnology , chemistry , isoelectric point , biology , biochemistry , enzyme
A new method of identifying allo A lectin‐binding glycoproteins separated by isoelectric focusing is described. The method is based on the combination of neuraminidase treatment, isoelectric focusing and Durapore filter blotting followed by detection with allo A lectin. The technique is simple and reproducible for the determination of both the alpha 1‐antitrypsin and orosomucoid phenotypes in genetic and forensic investigations.