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Tetramethylurea as a protein denaturing agent in gel electrophoresis
Author(s) -
Zazra James J.,
Szklaruk Boris
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080707
Subject(s) - tetramethylurea , electrophoresis , urea , chromatography , chemistry , bovine serum albumin , biochemistry , solvent
We have examined the utility of tetramethylurea as a protein denaturant in electrophoresis using transverse gradient gels. Its physical properties make it easier to work with than urea and it appears to denature bovine serum albumin in a similar way. Tetramethylurea is a potential substitute for urea in some electrophoretic systems.