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Rehydratable ultrasensitive cellulose acetate substrate films for protease detection in ultrathin‐layer isoelectric focusing not interfering with subsequent silver staining of focused proteins in the gel
Author(s) -
Kujat Richard
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080607
Subject(s) - isoelectric focusing , substrate (aquarium) , cellulose , chromatography , layer (electronics) , silver stain , protein detection , cellulose acetate , proteases , chemistry , protease , materials science , staining , nanotechnology , biochemistry , microbiology and biotechnology , enzyme , biology , ecology , genetics
A simple method of immobilizing protein on cellulose acetate by pretreating the films with methacryloxypropyltrimethoxysilane is presented. When a print is taken with such a substrate film the separating gel is not contaminated by the immobilized substrate, thus permitting location of focused proteins by means of silver stamina Dried films with immobilized substrate can be stored for several months before use Detection of alkaline and acid proteases is possible with a sensitivity of 0.3 ng/mm 2 .