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Reduced area two‐dimensional gels for direct digital imaging of radioactive protein profiles
Author(s) -
Ross Mary E.,
Latter Gerald,
Burbeck Stephen,
Leavitt John
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080510
Subject(s) - reproducibility , polyacrylamide , color marker , polyacrylamide gel electrophoresis , chromatography , spectrum analyzer , electrophoresis , chemistry , channel (broadcasting) , materials science , computer science , gel electrophoresis of proteins , optics , physics , computer network , biochemistry , polymer chemistry , enzyme
A method is demonstrated which reduces the area of a standard two‐dimensional polyacrylamide electrophoresis gel to approximately one‐fourth of its original area. Microdensitometry is used to show that the technique did not significantly alter the reproducibility of the gels. This reduction in size makes it possible to use a micro‐channel plate analyzer to directly digitize an entire gel.