Premium
A modified applicator strip for improved focusing on immobilized pH gradient gels
Author(s) -
Pflug Werner,
Laczko Bela
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080509
Subject(s) - isoelectric focusing , immobilized ph gradient , chromatography , strips , bovine serum albumin , isoelectric point , mixing (physics) , chemistry , concentration gradient , materials science , analytical chemistry (journal) , composite material , biochemistry , physics , quantum mechanics , enzyme
The use of applicator strips on isoelectric focusing in immobilized pH gradient gels often results in lateral sample spreading and mixing of adjacent samples. Both effects can be reduced or eliminated if a modified applicator strip with slits at the bottom is used. Different modes of sample application were compared using the group specific component system with different concentrations of bovine serum albumin in the samples.