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Anti‐mitochondrial protein antibodies in a serum from a patient with systemic lupus erythematosus: Specificity and comparison with other anti‐mitochondrial antibodies
Author(s) -
Celis Julio E.,
Madsen Peder,
Andersen Irene,
Andersen Paul,
Vayssiere JeanLuc,
Croizat Bernard,
Thiessen Hanne,
Nielsen Søren
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080507
Subject(s) - antibody , amnion , immunofluorescence , mitochondrion , microbiology and biotechnology , biology , cirrhosis , gel electrophoresis , lupus erythematosus , chemistry , immunology , medicine , biochemistry , fetus , pregnancy , genetics
Anti‐mitochondrial antibodies present in a serum from a systemic lupus erythematosus patient have been characterized by indirect immunofluorescence of cultured transformed human amnion cells, and by two‐dimensional gel electrophoresis and immunoblotting. The antibodies which stained mostly mitochondria in transformed human amnion cells reacted with four mouse brain cortex mitochondrial proteins. These proteins, which have been termed a, b, c and d, exhibited apparent molecular weights of 66 000, 49 000, 49 000 and 44 000, respectively. Proteins a and c exhibited charge variants. Surprisingly, most of the proteins recognized by this serum also reacted with sera from a patient with liver cirrhosis and idiopathic pulmonary fibrosis.