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Studies by conventional and “low denaturing” isoelectric focusing on albumin microheterogeneity under normal conditions and in experimental nephrosis
Author(s) -
Ghiggeri Gian M.,
Candiano Giovanni,
Queirolo Carlo,
Gusmano Rosanna,
Ginevri Fabrizio,
Bertani Tullio,
Remuzzi Giuseppe
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080502
Subject(s) - albumin , nephrotic syndrome , isoelectric focusing , nephrosis , chemistry , medicine , endocrinology , isoelectric point , gene isoform , serum albumin , urinary system , chromatography , urine , homogeneous , nephelometry , biochemistry , immunology , antibody , enzyme , physics , gene , thermodynamics
With the aid of ultrathin‐layer isoelectric focusing, rat serum albumin was studied in a group of 6 normal rats and 4 rats affected by an experimentally induced nephrotic syndrome. In normal rats the albumin was microheterogeneous with p I values ranging from 4.7 to 6.1. In animals with the nephrotic syndrome the albumin was almost homogeneous, consisting only of the isoform with the lowest p I of 4.7. The patterns were altered by performing IEF in low denaturing media in presence of 0.5–2 Murea. Under these conditions two rat serum albumin bands with p I 's close to 6.1 were observed. Urinary albumin was highly microheterogeneous in nephrotic rats (p I 4.7–6.1) and less heterogeneous in the normal group(p I 4.7). Pooled urinary albumin from nephrotic rats was fractionated by preparative IEF in granulated gels in four isoforms (A1–A4) with decreasing p I . Both for serum and urinary albumin the fatty acid content in normal and nephrotic syndrome animals was higher in isoforms with low p I .