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Improved separation of the common transferrin variants in gels containing pH 5–7 Ampholines and HEPES
Author(s) -
Budowle Bruce
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080407
Subject(s) - hepes , isoelectric focusing , immobilized ph gradient , chemistry , chromatography , transferrin , subtyping , isoelectric point , biochemistry , computer science , enzyme , programming language
An ultrathin‐layer polyacrylamide gel isoelectric focusing technique is described for obtaining highly resolved transferrin (Tf) subtypes. N‐2‐hydroxyethylpiperazine‐N′‐2‐ethanesulfonic acid (HEPES) buffer was used to flatten the pH gradient in gels containing pH 5–7 Ampholines. The result was increased separation distances for the TfC subtype bands. The method is effective even with the most recent lots of pH 5–7 Ampholines which were not capable of providing an appropriate environment for effective TfC subtyping.