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A simplification of Heukeshoven and Dernick's silver staining of proteins
Author(s) -
Damerval Catherine,
Guilloux Martine Le,
Blaisonneau Joël,
de Vienne Dominique
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080308
Subject(s) - silver nitrate , staining , distilled water , silver stain , sodium carbonate , polyacrylamide , chemistry , sodium , chromatography , polyacrylamide gel electrophoresis , nuclear chemistry , biochemistry , polymer chemistry , biology , organic chemistry , microbiology and biotechnology , enzyme , genetics
The procedure of Heukeshoven and Dernick for silver staining of proteins in polyacrylamide gels is simplified by applying Farmer's redücer before impregnation with silver nitrate and rinsing the gel in 2.5 % sodium carbonate instead of distilled water. This modification yields pattern of good contrast without a destaining‐restaining step.