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Enzyme polymorphism in bacteria: Study of molecular relatedness by combined isoelectric focusing‐electrophoresis
Author(s) -
Picard Bertrand,
Krishnamoorthy Rajagopal,
Goullet Philippe
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080306
Subject(s) - isoelectric focusing , isoelectric point , electrophoresis , malate dehydrogenase , biology , biochemistry , enzyme , microbiology and biotechnology , acid phosphatase , polymorphism (computer science) , chemistry , gene , genotype
The molecular relationships between electrophoretic variants of different bacteria, defined in two‐dimensional electrophoretic profiles of acid phosphatase, esterases, glutamate dehydrogenase and malate dehydrogenases, were analyzed by combined isoelectric focusing‐electrophoresis. The mobility curves of enzymes confirmed the monomorphism of variants showing identical isoelectric point (p I ) and relative electrophoretic mobility (M F ) values and refined the polymorphism analysis of variants showing distinct p I and/or M F values.