Premium
Cyanogen bromide‐activated nitrocellulose membranes: A new tool for immunoprint techniques
Author(s) -
Demeulemester Claude,
Peltre Gabriel,
Laurent Micheline,
Panheleux Dominique,
David Bernard
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080113
Subject(s) - nitrocellulose , collodion , dactylis glomerata , cyanogen bromide , chromatography , isoelectric focusing , electroblotting , membrane , coomassie brilliant blue , chemistry , agarose , staining , biochemistry , biology , botany , genetics , poaceae , gene , peptide sequence , enzyme
Nitrocellulose membranes were activated by cyanogen bromide in order to improve their binding capacities. Crude extracts of a grass pollen, Dactylis glomerata , and a mite, Dermatophagoides farinae , were separated by isoelectric focusing in agarose and blotted by capillary transfer onto nitrocellulose or activated nitrocellulose. The Dermatophagoides farinae components were visualized by Coomassie Brilliant Blue, India ink or Aurodye staining. The Dactylis glomerata and Dermatophagoides farinae allergens were revealed by radioimmunodetection using sera from allergic patients. A great increase of allergen binding was observed visually and by radioactive counting on activated nitrocellulose as compared with untreated nitrocellulose. New allergens which were not detectable on nitrocellulose appeared on activated nitrocellulose.