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A new method for sex determination of the donor of forensic samples using a recombinant DNA probe
Author(s) -
Gill Peter
Publication year - 1987
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150080108
Subject(s) - recombinant dna , dna , microbiology and biotechnology , blood stains , biology , chemistry , chromatography , genetics , gene
Microscopical methods for sex determination of denatured samples in forensic laboratories are frequently unreliable. This paper describes the use of a recombinant DNA probe which hybridises specifically to a 2.47 kb repeat sequence of which 2000 copies are present on the Y chromosome. The technique enables rapid and reliable sex determinations of degraded DNA samples and produces results from blood stains greater than 4 years old in which only low molecular weight (< 10 kb) DNA is present. Stains from less than 1 μL of blood or a single hair root is required for the test using dot blot hybridisations. Electrophoretic analyses revealed that Hae III restriction of DNA samples produced somatically stable patterns.