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Immobilized pH gradients in capillary tubes and two‐dimensional gel electrophoresis
Author(s) -
Hochstrasser Denis,
Augsburger Valérie,
Funk Matthieu,
Appel Ron,
Pellegrini Christian,
Muller Alex F.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150071105
Subject(s) - isoelectric focusing , immobilized ph gradient , chromatography , capillary electrophoresis , electrophoresis , chemistry , resolution (logic) , free flow electrophoresis , two dimensional gel electrophoresis , isoelectric point , gel electrophoresis of proteins , capillary action , analytical chemistry (journal) , polyacrylamide gel electrophoresis , materials science , biochemistry , computer science , proteomics , composite material , artificial intelligence , gene , enzyme
Abstract Isoelectric focusing in mixed carrier ampholyte‐immobilized pH gradients (CA‐IPG) is an effective way to separate proteins by charge. A method to prepare CA‐IPG in capillary tubes and the description of the equipment used are outlined. Two‐dimensional gel electrophoresis patterns of human serum and liver biopsies, with isoelectric focusing and CA‐IPG as the first dimension, are presented. The comparison with two‐dimensional gel electrophoresis patterns obtained by conventional carrier ampholyte pH gradient separation shows the excellent resolution and potential of this technique.