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Computer simulation of two electrophoretic columns coupled for isoelectric focusing in simple buffers
Author(s) -
Tsai Amos,
Mosher Richard A.,
Bier Milan
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150071102
Subject(s) - isoelectric focusing , electrophoresis , electrolyte , anode , chemistry , column (typography) , chromatography , cathode , buffer (optical fiber) , isoelectric point , analytical chemistry (journal) , mixing (physics) , constant (computer programming) , current (fluid) , thermodynamics , computer science , physics , electrode , telecommunications , biochemistry , frame (networking) , quantum mechanics , programming language , enzyme
Computer simulation is used to analyze a system of two electrophoretic columns coupled by mixing the anolyte of one with the catholyte of the other. A mathematical model is presented which is used to predict the pH gradients formed by monovalent buffers in this system, when the currents in the columns are unequal. In the column with the higher current a pH gradient is created which increases from anode to cathode and is potentially useful for isoelectric focusing. The breadth of this gradient is dependent upon the ratio of the currents. The function of the second column is the compensation of buffer migration which occurs in the first column, thereby maintaining constant electrolyte composition. The effects of buffer p K s and mobilities are evaluated.