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Reducing the background to silver‐stained polyacrylamide gels after two‐dimensional electrophoresis
Author(s) -
Amess Robert,
Peter Spragg S.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150071003
Subject(s) - silver stain , staining , polyacrylamide , polyacrylamide gel electrophoresis , electrophoresis , chromatography , chemistry , gel electrophoresis , materials science , microbiology and biotechnology , biology , biochemistry , polymer chemistry , genetics , enzyme
A high background found after silver staining of polyacrylamide gels following two‐dimensional electrophoresis and generated from the cathode in the second‐dimensional separation has been investigated. Material is formed which is electrophoretically mobile and apparently derived from the buffer components. It behaves like a peptide as far as silver staining. The intensity of this background was reduced by treatment of the second‐dimensional gel with a photographic fixing agent prior to silver staining. Moreover, spots which previously appeared negatively stained were changed by the treatment and the result was a general improvement in the contrast.