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A novel method for permanent imprints from dye‐stained electrophoretograms
Author(s) -
Ramasamy Muthiah,
Wimmer Dave H.,
Kurup Viswanath P.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150071002
Subject(s) - staining , chromatography , coomassie brilliant blue , chemistry , biology , genetics
A simple method for obtaining permanent imprints of Coomassie Blue and Amido Black stained electrophoretograms on developed unexposed photographic paper and film is described. Copies of slab and two‐dimensional electrophoretograms and crossed immunoelectrophoretograms were obtained by this technique. The method is fast, simple, inexpensive and can be adapted to make as many as five copies by repeated staining of the electrophoretogram. The copies are the exact size of the original gels and can be stored for a long time. The possible use of these imprints for the comparative study of immunoblots with the corresponding protein‐stained gels is discussed.