Urine analysis by two‐dimensional gel eletrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension | Zendy

Gianazza Elisabetta | Zendy; AstruaTestori Silvia | Zendy; Righetti Pier Giorgio | Zendy; BianchiBosisio Adriana | Zendy

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Urine analysis by two‐dimensional gel eletrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension

Author(s) -

Gianazza Elisabetta,

AstruaTestori Silvia,

Righetti Pier Giorgio,

BianchiBosisio Adriana

Publication year - 1986

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150070911

Subject(s) - isoelectric focusing , chromatography , isoelectric point , chemistry , urine , electrophoresis , immobilized ph gradient , biochemistry , enzyme

Urine analysis by two‐dimensional gel electrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension allows the loading of high sample volumes and/or of large amounts of salts. When applied to urinary protein analysis, it makes it possible to apply up to 1 mL of dialyzed, unconcentrated sample or up to 350 μL of unprocessed urine.

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