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Urine analysis by two‐dimensional gel eletrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension
Author(s) -
Gianazza Elisabetta,
AstruaTestori Silvia,
Righetti Pier Giorgio,
BianchiBosisio Adriana
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070911
Subject(s) - isoelectric focusing , chromatography , isoelectric point , chemistry , urine , electrophoresis , immobilized ph gradient , biochemistry , enzyme
Urine analysis by two‐dimensional gel electrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension allows the loading of high sample volumes and/or of large amounts of salts. When applied to urinary protein analysis, it makes it possible to apply up to 1 mL of dialyzed, unconcentrated sample or up to 350 μL of unprocessed urine.