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On‐line fiber optic UV detection cell and conductivity cell for capillary zone electrophoresis
Author(s) -
Foret František,
Deml Mirko,
Kahle Vladislay,
Boček Petr
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070909
Subject(s) - capillary electrophoresis , capillary action , isotachophoresis , conductivity , materials science , absorbance , optical fiber , electrolyte , analytical chemistry (journal) , chromatography , optoelectronics , optics , chemistry , electrode , composite material , physics
Simple detection cells for on‐line UV absorbance and conductivity detection in capillary zone electrophoresis are described. The UV detection system employs a commercial photometer and optical fibers which are in direct contact with the outer walls of the separation capillary and conduct the light beam perpendicularly across the migrating zones. The conductivity detection cell consists of platinum wires protruding the wall of the separation capillary and thus in direct contact with the electrolytes inside. The electronic system is adopted from a commercially available conductivity detector for isotachophoresis.