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Efficient protein blotting using an economical power supply
Author(s) -
Fischer Itzhak
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070908
Subject(s) - blot , nitrocellulose , power (physics) , chemistry , chromatography , computer science , biochemistry , physics , quantum mechanics , membrane , gene
A simple and inexpensive system of a power supply for protein blotting is described. The blots are run using a 12 V car battery charger which is modified for laboratory use. Under these conditions even high molecular weight proteins of 300 000 can be completely transferred to the nitrocellulose paper in less than 5 h and efficiently immunostained.
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