Premium
The condensation of ampholytes in steady state moving boundaries: Analysis by computer simulation
Author(s) -
Mosher Richard A.,
Thormann Wolfgang
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070902
Subject(s) - steady state (chemistry) , chemistry , condensation , cationic polymerization , isoelectric focusing , conductivity , sample (material) , electrolyte , biological system , state (computer science) , chemical physics , mechanics , analytical chemistry (journal) , thermodynamics , chromatography , computer science , physics , algorithm , polymer chemistry , electrode , biology , enzyme , biochemistry
A digital simulation of the behavior of amphoteric sample components in moving steady state boundaries is presented. Complete computer simulation data, including profiles of concentration, conductivity and pH as functions of time, are given for both cationic and anionic electrolyte configurations which incorporate one amphoteric sample constituent. The condensation of ampholytes in steady state moving boundaries is shown to proceed via an isotachophoretic mechanism and not by isoelectric focusing. Mobility (velocity) relationships necessary for sample components to form steady state zones are discussed.