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Detection of soluble peroxidases by direct plant tissue isoelectric focusing
Author(s) -
Vioque Blanca,
López Antonio,
Castellano José M.,
Albi Miguel A.,
Vioque Agustín
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070811
Subject(s) - isoelectric focusing , distilled water , polyacrylamide , isoelectric point , peroxidase , aqueous solution , chromatography , chemistry , potassium , polyacrylamide gel electrophoresis , electrophoresis , biochemistry , polymer chemistry , enzyme , organic chemistry
Rectangular sections of leaves, vacuum‐infiltrated with distilled water or aqueous solutions of potassium chloride, were applied directly onto the surface of polyacrylamide gels followed by isoelectric focusing. Soluble peroxidases (free and ionically bound) were released from the tissue on isoelectric focusing with resultant characteristic zymograms for the different plant tissues.