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Galactokinase: Technique for polacrylamide gel isoelectric focusing
Author(s) -
Stambolian Dwight
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070810
Subject(s) - galactokinase , isoelectric focusing , chromatography , ideal (ethics) , chemistry , biochemistry , enzyme , philosophy , epistemology , escherichia coli , gene
A new technique is presented for the detection of human galactokinase from crude hemolysate by the method of polyacrylamide gel isoelectric focusing. The method utilizes low sample volume and can be completed within 5 h. One gel can be used to screen 15 samples, making the procedure ideal for screening erythrocyte galactokinase in newborn screening programs. This method can be ideal for many laboratories interested in screening for galactokinase variants.