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A novel method for molecular weight determination in immunoblots
Author(s) -
Hohmann Art,
Lemon Jodie,
Nikoloutsopoulous Tony
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070809
Subject(s) - antiserum , sodium dodecyl sulfate , blot , chromatography , gel electrophoresis , polyacrylamide gel electrophoresis , membrane , chemistry , electrophoresis , microbiology and biotechnology , biochemistry , antibody , biology , gene , immunology , enzyme
A novel method is described to serve as a positive control by including an antiserum raised against and reacted with blots of the molecular standards used in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The marker antiserum may find further application on nylon based adsorptive membranes which cannot be stained by conventional methods.